BAMFI ENDONUCLEASE Meaning and
Definition
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BamHI Endonuclease is a type II restriction enzyme that is commonly used in molecular biology and genetic engineering techniques. It is derived from the bacterium Bacillus amyloliquefaciens H, from which it gets its name.
Endonucleases are enzymes capable of breaking the phosphodiester bonds within a nucleic acid molecule, resulting in the cleavage of the molecule at specific sequence patterns. BamHI endonuclease specifically recognizes and cleaves the DNA sequence 5'-GGATCC-3'. The cleavage occurs between the two G residues, generating DNA fragments with single-stranded overhangs called "sticky ends". These sticky ends can then be joined with complementary sticky ends from other DNA molecules, allowing for the insertion of foreign DNA sequences into a plasmid or other vectors.
BamHI endonuclease is widely used in recombinant DNA technology for purposes such as gene cloning, DNA sequencing, and genetic engineering. Its predictable and specific cleavage pattern makes it an essential tool for manipulating DNA fragments. The enzyme is commercially available and can be used in various experimental procedures in molecular biology laboratories.
Overall, BamHI endonuclease plays a crucial role in the field of genetic engineering as it enables the precise manipulation and recombination of DNA molecules, allowing researchers to explore and modify genetic information in a controlled and targeted manner.
Common Misspellings for BAMFI ENDONUCLEASE
- vamfi endonuclease
- namfi endonuclease
- hamfi endonuclease
- gamfi endonuclease
- bzmfi endonuclease
- bsmfi endonuclease
- bwmfi endonuclease
- bqmfi endonuclease
- banfi endonuclease
- bakfi endonuclease
- bajfi endonuclease
- bamdi endonuclease
- bamci endonuclease
- bamvi endonuclease
- bamgi endonuclease
- bamti endonuclease
- bamri endonuclease
- bamfu endonuclease
- bamfj endonuclease
- bamfk endonuclease
Etymology of BAMFI ENDONUCLEASE
The term "BamHI endonuclease" is derived from the name of the bacterium species it was first isolated from (Bacillus amyloliquefaciens) and the type II restriction enzyme it belongs to (restriction endonuclease). The "Bam" in BamHI stands for Bacillus amyloliquefaciens, while the "H" indicates that it was the first restriction enzyme isolated from that bacterium. The "I" refers to the order of discovery among restriction enzymes from Bacillus amyloliquefaciens. Hence, "BamHI endonuclease" is named after the bacterium it was derived from and its identification as a specific endonuclease.
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