AACI ENDONUCLEASE Meaning and
Definition
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AacI Endonuclease is an enzyme that belongs to the endonuclease family, specifically the type II restriction endonuclease group. It is derived from the bacterium Aquifex aeolicus. AacI Endonuclease is classified as a restriction enzyme due to its ability to recognize a specific DNA sequence and cleave the DNA at that site.
The recognition sequence for AacI Endonuclease is 5'-AAC(↓)GTT-3', where the downward arrow indicates the site of cleavage. This palindromic sequence indicates that the same sequence is read in the 5' to 3' direction on both DNA strands. Once the recognition sequence is identified, AacI Endonuclease binds to the DNA and catalyzes the hydrolysis of the DNA phosphodiester bonds, creating a double-strand break.
AacI Endonuclease plays a crucial role in molecular biology research, particularly in DNA manipulation techniques such as cloning and genetic engineering. By cleaving DNA at specific sites, it allows for the isolation, purification, and manipulation of specific DNA fragments. This enzyme is often used in conjunction with other restriction enzymes to generate cohesive ends or blunt ends, depending on the desired application.
Overall, AacI Endonuclease is a type II restriction endonuclease that recognizes and cleaves DNA at the 5'-AAC(↓)GTT-3' sequence. Its specific cleavage activity provides a valuable tool in molecular biology research for DNA manipulation and analysis.
Common Misspellings for AACI ENDONUCLEASE
- zaci endonuclease
- saci endonuclease
- waci endonuclease
- qaci endonuclease
- azci endonuclease
- asci endonuclease
- awci endonuclease
- aqci endonuclease
- aaxi endonuclease
- aavi endonuclease
- aafi endonuclease
- aadi endonuclease
- aacu endonuclease
- aacj endonuclease
- aack endonuclease
- aaco endonuclease
- aac9 endonuclease
- aac8 endonuclease
- aaci wndonuclease
- aaci sndonuclease
Etymology of AACI ENDONUCLEASE
The word "AacI Endonuclease" consists of two parts: "AacI" and "endonuclease".
1. AacI: The term "AacI" refers to a specific restriction enzyme named AacI. Restriction enzymes are proteins that can recognize specific DNA sequences and cut the DNA at those sites. The "Aac" part of AacI represents the organism from which the enzyme was isolated, which is typically abbreviated for convenience. In this case, "Aac" stands for Arthrobacter luteus, a species of bacteria.
2. Endonuclease: The term "endonuclease" is a combination of two Latin roots: "endo-" meaning "within" or "inside", and "-nuclease" which refers to an enzyme that breaks down nucleic acids (DNA or RNA) by cutting them into smaller fragments.
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